Oligonucleotide‐directed, site‐specific mutagenesis is being applied to the problem of ion‐gradient driven active transport with the lac permease as a model system. It has been shown that Arg‐302, His‐322 and Glu‐325, neighboring residues in putative transmembrane helices IX and X, play an important role in lactose‐coupled H+ translocation, possibly as components of a catalytic triad similar to that found in the serine proteases. In addition, Cys residues, long thought to be involved in the mechanism of the permease, are not directly (...) involved in either substrate binding or H+ translocation. Finally, a variety of mutations have no effect on permease activity indicating that single amino acid changes do not lead indiscriminately to long‐range conformational alterations. (shrink)

The Political Crisis and Christian Ethics addresses themes in political philosophy in the context of a crisis in democracy after the denial of the 2020 election by the Republican candidate for president. The refusal to accept the results of the election divided the electorate and drove the president's followers to fail in their attempted coup attempt in January of 2020. Democracy is defended in Reinhold Niebuhr's writing on politics and in Barack Obama's use of the theologian's thought. It is developed (...) further in the political theory of Paul Tillich. The themes of just peacemaking are reviewed in Paul Tillich's critique of John Foster Dulles' work and in the author's critique of just peacemaking in the work of Glen Stassen. Domestically the issues of race, inequality, ecology, and healthcare are addressed from the perspective of prophetic realism. The book concludes in terms of Alfred North Whitehead's philosophy of education and religion and a vision of the good president. In summary, The Political Crisis and Christian Ethics is a volume of American, Christian political theory in a period of overcoming the trauma of 2016 with Christian ethics and political philosophy. (shrink)

Somatic cell gene therapy has yielded promising results. If germ cell gene therapy can be developed, the promise is even greater: hundreds of genetic diseases might be virtually eliminated. But some claim the procedure is morally unacceptable. We thoroughly and sympathetically examine several possible reasons for this claim but find them inadequate. There is no moral reason, then, not to develop and employ germ-line gene therapy. Taking the offensive, we argue next that medicine has a prima facie moral obligation to (...) do so. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:THE QUEST FOR AN ADEQUATE PROPORTIONALIST THEORY OF VALUE RoNALD H. McKINNEY, S.J. U'IWversity of Scranton Scranton, Pennsylvania EDWARD VACEK shrewdly observes that proportionalism attempts to synthesize the crucial insights of both the teleologist and the deontologist.1 Indeed, Vacek provides a fine summary of this achievement. However, he reflects that the most underdeveloped feature of proportionalism is its value theory by which we are enabled to know how (...) to resolve conflict situations.2 Richard McCormick, among others, has attempted to meet this deficiency by ·adopting John Finnis' notion of associated basic goods. In this essay, I will show how such an ·approach fails to remain true to the basic aims of proportionalism. Nor should this come as a surprise, since Finnis is himself indebted to one of McCormick's archopponents, Germain Grisez. Moreover, I will attempt to outline the elements involved in a non-hierarchfoal theory of value which I will argue to be the only adequate alternative to McCormick 's attempted solution. Deontologists argue that certain acts are right or wrong regardless of the ·consequences. Proportionalists, however, are opposed to such notions as an " intrinsically evil " act or an "absolute" duty, since they recognize the existence of moral dilemmas. A moral dilemma arises whenever there is a situation in which pre-moral values •are in conflcit, i.e., whatever choice one makes will result in the omission or harm of one or other of the values at stake. According to the proportionl Edward Vacek, "Proportionalism: One View of the Debate," Theological Studies 46/2 (June, 1985), 289. 2 Va.eek, 302. 56 PROPORTIONALIST THEORY OF VALUE 57 alist reformulation of the principle of double effect, the permission or oausrution of ontic evil is not necessarily a moral evil as long as there is a proportionate reason justifying,such an action, e.g., when a father shoots an!intruder threatening to harm his family. McCormick's initial description of the requirements of proportionate reason [s ias follows: "a) a v1alue at least equal to that sacrificed is at 1 stake; b) there is no less harmful way of protecting ithe value here and now; c) the manner of its protection here and now will not undermine it in the long run." 8 McCormick argues that it simply will not do to claim that the ontic evil permitted or caused in a conflict situation is always only indirectly intended.4 For, in many instances, this simply is not true. Moreover, he would argue that to intend an end is not necess1arily to desire it as deontologists suppose. What justifies the ontic evil resulting from such actions, then, is solely the proportionate reason for which it is allowed or caused. There are some obvious criticisms that have been hurled at this proportionalist proposal for resolving conflict situations: I) that it leaves itself open to the practical, if not theoretical, difficulty of weighing different values;!2) that it results in the possibility of many resolutions that could go counter to our normal moral intuitions; 8) that there exists an inherent fallibility in the calcul,ation of long-range consequences; and 4) that this uncertainty might easliy promote an undesirable attitude of skeptical relativism. It is perhaps the onslaught of these and other criticisms which prompted McCormick to adopt Finnis' theory of associated basic goods. Lisa Cahill,argues that this new theory constitutes an "abandonment" of McCormick's prior proposal.5 To understand how this new theory leads to inevitable s Richard McCormick, ".Ambiguity in Moral Choice," Doing Evil To Achieve Good: Moral Choice in Oonfiict Situations, eds. R. McCormick and P. Ramsey (Chicago: Loyola U. Press, 1978), 45. 4 McCormick, 35-38. 5 Lisa Cahill, "Theology, Utilitarianism, and Christian Ethics," Theologica,I Studies 42/4 (Dec., 1981), 618-24. 58 RONALD H. MCKINNEY, S.J. inconsistency for McCormick, we must first examine Finnis' position itself. Finnis' Theory of Basic Goods By "value," Finnis refers to any " general form of good that oan be participated iin or realized in indefinitely many ways on indefinitely many occasions." 6 Basic values, moreover, refer to ·intrinsic goods that are desired for their own sake (NL, 62, 65). They are good not because they are... (shrink)

The discovery of induced pluripotent stem (iPS) cells in 2006 was heralded as a major breakthrough in stem cell research. Since then, progress in iPS cell technology has paved the way towards clinical application, particularly cell replacement therapy, which has refueled debate on the ethics of stem cell research. However, much of the discourse has focused on questions of moral status and potentiality, overlooking the ethical issues which are introduced by the clinical testing of iPS cell replacement therapy. First-in-human trials, (...) in particular, raise a number of ethical concerns including informed consent, subject recruitment and harm minimisation as well as the inherent uncertainty and risks which are involved in testing medical procedures on humans for the first time. These issues, while a feature of any human research, become more complex in the case of iPS cell therapy, given the seriousness of the potential risks, the unreliability of available animal models, the vulnerability of the target patient group, and the high stakes of such an intensely public area of science. Our paper will present a detailed case study of iPS cell replacement therapy for Parkinson's disease to highlight these broader ethical and epistemological concerns. If we accept that iPS cell technology is fraught with challenges which go far beyond merely refuting the potentiality of the stem cell line, we conclude that iPS cell research should not replace, but proceed alongside embryonic and adult somatic stem cell research to promote cross-fertilisation of knowledge and better clinical outcomes. (shrink)

St. Augustine is the bridge that links ancient philosophy and early Christian theology to the thought patterns of the Middle Ages. But the influence of Augustine's philosophy in general and his epistemology in particular extends far beyond medieval philosophy. Such modern philosophers as Descartes and Malebranche carry the stamp of Augustinism upon their philosophies. What is not so well known is that even some of the most original ideas of Berkeley and Kant can be found anticipated in Augustine.

This first book-length study of Paul Tillich's ethics is drawn from research in the Harvard Archives and fifty years of teaching Tillich's social-political thought. In Ronald H. Stone's fourth work on Tillich's philosophy the ethic is examined from the early ontological to socialist ethics to his own final principled-situationalist ethic in late life. Unique to this study is the in-depth inquiry into Tillich's courageous social action correlated with his own philosophical-theological ethic. The book moves from an early socialist rally (...) in Berlin, through the wars, dialogue with John Foster Dulles about post-war planning, debates about nuclear deterrence, to Buddhist Christian dialogue. The author's own preference for the late ethic of the philosopher informs the inquiries into the earlier radical Tillich. The conclusion provides a synthesis of the vast sources of Tillich's ethics and presents twelve themes summarizing sources and future resources for ethics from his life's work. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:THE QUEST FOR AN ADEQUATE PROPORTIONALIST THEORY OF VALUE RoNALD H. McKINNEY, S.J. U'IWversity of Scranton Scranton, Pennsylvania EDWARD VACEK shrewdly observes that proportionalism attempts to synthesize the crucial insights of both the teleologist and the deontologist.1 Indeed, Vacek provides a fine summary of this achievement. However, he reflects that the most underdeveloped feature of proportionalism is its value theory by which we are enabled to know how (...) to resolve conflict situations.2 Richard McCormick, among others, has attempted to meet this deficiency by ·adopting John Finnis' notion of associated basic goods. In this essay, I will show how such an ·approach fails to remain true to the basic aims of proportionalism. Nor should this come as a surprise, since Finnis is himself indebted to one of McCormick's archopponents, Germain Grisez. Moreover, I will attempt to outline the elements involved in a non-hierarchfoal theory of value which I will argue to be the only adequate alternative to McCormick 's attempted solution. Deontologists argue that certain acts are right or wrong regardless of the ·consequences. Proportionalists, however, are opposed to such notions as an " intrinsically evil " act or an "absolute" duty, since they recognize the existence of moral dilemmas. A moral dilemma arises whenever there is a situation in which pre-moral values •are in conflcit, i.e., whatever choice one makes will result in the omission or harm of one or other of the values at stake. According to the proportionl Edward Vacek, "Proportionalism: One View of the Debate," Theological Studies 46/2 (June, 1985), 289. 2 Va.eek, 302. 56 PROPORTIONALIST THEORY OF VALUE 57 alist reformulation of the principle of double effect, the permission or oausrution of ontic evil is not necessarily a moral evil as long as there is a proportionate reason justifying,such an action, e.g., when a father shoots an!intruder threatening to harm his family. McCormick's initial description of the requirements of proportionate reason [s ias follows: "a) a v1alue at least equal to that sacrificed is at 1 stake; b) there is no less harmful way of protecting ithe value here and now; c) the manner of its protection here and now will not undermine it in the long run." 8 McCormick argues that it simply will not do to claim that the ontic evil permitted or caused in a conflict situation is always only indirectly intended.4 For, in many instances, this simply is not true. Moreover, he would argue that to intend an end is not necess1arily to desire it as deontologists suppose. What justifies the ontic evil resulting from such actions, then, is solely the proportionate reason for which it is allowed or caused. There are some obvious criticisms that have been hurled at this proportionalist proposal for resolving conflict situations: I) that it leaves itself open to the practical, if not theoretical, difficulty of weighing different values;!2) that it results in the possibility of many resolutions that could go counter to our normal moral intuitions; 8) that there exists an inherent fallibility in the calcul,ation of long-range consequences; and 4) that this uncertainty might easliy promote an undesirable attitude of skeptical relativism. It is perhaps the onslaught of these and other criticisms which prompted McCormick to adopt Finnis' theory of associated basic goods. Lisa Cahill,argues that this new theory constitutes an "abandonment" of McCormick's prior proposal.5 To understand how this new theory leads to inevitable s Richard McCormick, ".Ambiguity in Moral Choice," Doing Evil To Achieve Good: Moral Choice in Oonfiict Situations, eds. R. McCormick and P. Ramsey (Chicago: Loyola U. Press, 1978), 45. 4 McCormick, 35-38. 5 Lisa Cahill, "Theology, Utilitarianism, and Christian Ethics," Theologica,I Studies 42/4 (Dec., 1981), 618-24. 58 RONALD H. MCKINNEY, S.J. inconsistency for McCormick, we must first examine Finnis' position itself. Finnis' Theory of Basic Goods By "value," Finnis refers to any " general form of good that oan be participated iin or realized in indefinitely many ways on indefinitely many occasions." 6 Basic values, moreover, refer to ·intrinsic goods that are desired for their own sake (NL, 62, 65). They are good not because they are... (shrink)

It is somewhat ironic that animals that are the prime choice for detailed genetic analysis, such as the fruit fly and the nematode, have thus far been largely refractory to reverse genetic analysis. Their detailed genetic map, and small genome size have made them subjects of ambitious genome analysis projects, but there is still no strategy to introduce desired changes into their genomes by homologous recombination. Some alternative approaches have recently become available; this review describes possibilities and unsolved problems for (...) reverse genetics in the nematode Caenorhabditis elegans. The transposon Tc1 could prove to be very useful for the isolation of knock out mutants, and possibly also for introduction of more subtle alterations. (shrink)

"In an age when objective moorings are being cut loose and experience reigns supreme, we need more than ever to reiterate that the distinctive feature of Christianity is its grounding in history. In this concise, well-written work, a noted philosopher and committed evangelical enables thoughtful readers to grapple with key questions in the relationship between faith and historical understanding and leads them to the awareness of a necessity for commitment to the One who stands behind as well as in history." (...) ? Richard V. Pierard, Professor of History, Indiana State University "Highly recommended." ? Calvin Theological Journal "Professor Nash has given us a lucid exposition of an important subject that should concern both Christian and serious inquirers into the basis of Christianity. He has the gift of making very profound philosophical issues comprehensible to the average reader. His use of quotations and illustrations is most illuminating. I appreciated especially his clear analysis of the existential and naturalistic presuppositions of a key New Testament scholar, Rudolf Bultmann. I will be glad to recommend this volume to undergraduates, graduates, and faculty colleagues." ? Edwin Yamauchi, Former President, Conference on Faith and History Professor, Miami University, Oxford, Ohio. (shrink)

Life's Ultimate Questions is unique among introductory philosophy textbooks. By synthesizing three distinct approaches—topical, historical, and worldview/conceptual systems—it affords students a breadth and depth of perspective previously unavailable in standard introductory texts. Part One, Six Conceptual Systems, explores the philosophies of: naturalism, Plato, Aristotle, Plotinus, Augustine, and Aquinas. Part Two, Important Problems in Philosophy, sheds light on: The Law of Noncontradiction, Possible Words, Epistemology I: Whatever Happened to Truth?, Epistemology II: A Tale of Two Systems, Epistemology III: Reformed Epistemology, God (...) I: The Existence of God, God II: The Nature of God, Metaphysics: Some Questions About Indeterminism, Ethics I: The Downward Path, Ethics II: The Upward Path, Human Nature: The Mind-Body Problem and Survival After Death. (shrink)

The Ultimate Imperative reclaims the love ethic as expressed in principles from the Ten Commandments and Jesus. Ronald Stone sets this ethic in tension with more recent theological insights and church pronouncements in order to explore personal and social issues, including race relations, economics, politics, ecology, and peacemaking.

Beginning from a basis in the theoretical analysis of comparative religious ethics provided by David Little and Sumner Twiss, this essay extends that analysis by sketching certain "benchmark" theoretical options in comparative religious ethics and by identifying certain fundamental questions which ethicists ought to address to the data supplied by descriptive studies of comparative religions. To illustrate the application of the theoretical model thus defined, the essay concludes with an analysis of selected themes in the essays by Campany, Guberman, and (...) Read in this Focus. (shrink)

The title of this book can be understood in at least two ways. First of all, The Word of God and the Mind of Man is an exploration of the extent to which the human mind can receive and understand divine revelation, insofar as this revelation is understood to include the communication of truth. On a second and more fundamental level, the phrase the word of God recalls its classical context -- the prologue to John's Gospel and the classical Logos (...) doctrine of the early church fathers: all human knowledge is possible because of the unique human participation in the eternal Logos of God, Jesus Christ. - Preface. (shrink)

Stone argues that religion must be understood in its connections with world politics for the successful conduct of foreign policy. Without peace among religions, there can be no peace, and without understanding the role of religion in politics, there can be neither peace nor successful foreign policy.